My target audience are people "on the fence" of becoming transphobic, and people who cause harm to the trans community without knowing it by spreading misinformation (that they don't know is misinformation) and disrespecting trans people overall.
Right now, I just want a list of transphobic claims to debunk, and ideally sources to aid in debunking them.
While I won't be going through the list point-for-point in the video essay, I intend to slot them into relevant sections that I've already planned out.
Reliable experts to research for reference would also be very helpful. 
My goal is to be as non-hostile as possible--extending the courtesy of respect, in the hopes that some of them will reciprocate that respect.
I don't expect civility from the entire group, but every new ally counts. 
I've been following this research document for awhile as I watched nominal.naomi's videos
https://www.masterdoc.info/
and
https://docs.google.com/document/d/1WZBpR9Ll3lNi7-ig8FvB2grMlhsmRZNa34cROGK2rEE