My target audience are people "on the fence" of becoming transphobic, and people who cause harm to the trans community without knowing it by spreading misinformation (that they don't know is misinformation) and disrespecting trans people overall.
Right now, I just want a list of transphobic claims to debunk, and ideally sources to aid in debunking them.
While I won't be going through the list point-for-point in the video essay, I intend to slot them into relevant sections that I've already planned out.
Reliable experts to research for reference would also be very helpful. 
My goal is to be as non-hostile as possible--extending the courtesy of respect, in the hopes that some of them will reciprocate that respect.
I don't expect civility from the entire group, but every new ally counts. 
For anything regarding the (usually extremely concern-trolly) issue of women's sports, this production has a lot of good resources.
https://www.tested-podcast.com/
Regret/dissatisfaction rate for gender affirming surgeries is close to 1%.
https://pmc.ncbi.nlm.nih.gov/articles/PMC8099405/
This figure is much lower than the same metric for other surgeries.
https://theconversation.com/transgender-regret-research-challenges-narratives-about-gender-affirming-surgeries-220642 (contains several links to journal articles)