From the research paper:

a) Comparison of wt and mRFP-modified major ampullate silk fibers rolled on a capillary glass (scale bars: 550 µm).

b) Strong red fluorescence can also be seen in the major ampullate gland (scale bar: 277 µm).

c i) The genomic implementation of mRFP into the major ampullate silk was confirmed by amplifying the mRFP DNA sequence extracted from the spider's leg. Only those spiders with red fluorescent silk (scale bar: 138 µm) showed the mRFP sequence-derived signal in the agarose gel.

C ii) Total-RNA was extracted from the glands, reverse-transcribed, and subjected to R-TqPCR and a melting curve analysis showing a peak at 83°C and 87°C based on a small and a large, amplified fragment.