For me, it would be GLaDOS.
Portal 2 spoiler
She deleted the one remaining human part of herself because it was just too much to deal with. She releases you at the end of Portal 2 as a path of least resistance measure because "killing you is hard."
What about you, what is your favorite media villain?
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Logo design credit goes to: tubbadu
Long John Silver from Treasure Island. The OG and best anti-hero and heel turn of any character.
Miss Havisham from Great Expectations . An textbook example of how to do capricious rot.
Baby Jane played by Bette Davis from 'Whatever Happened to Baby Jane?'. God I love Bette Davis.
Traitor General from the comic Rogue Trooper. Traitor General has been a wonderful bastard over the years.
Arthur 'Cody' Jarrett played by James Cagney from 'White Heat'.
Clarence Bodicker played by Kurtwood Smith from 'Robocop'. "Can you fly, Bobby?" 'Nuff said.
Daimyo Matsudaira Naritsugu played by Goro Inagaki in Takashi Miike's '13 Assassins'. Perfect psychotic aristo upheld by corrupt flunkeys who has no real conception of the effects of his actions.
Prince Nuada played by one of the Bros twins from 'Hellboy II'. So sympathetic. Team Prince Nuada all the way.
Syd played by Peter Mullan from 'Children of Men'. Syd refers to himself in the third person (An actual sign of psychopathy) and does not care about anyone else, not even the first pregnant woman in a decade..
Begbie played by Robert Carlyle from 'Trainspotting'. "Nae cunt leaves here til we find oot what cunt glassed yon lassie." Never mind that he threw the glass.