Please don't do this. This is just misgendering by default. The vast majority of people are exactly the gender they appear to be on the surface, and if they aren't, they'll let you know. I've only known one person who wasn't the gender they appeared (a very masculine-presenting enby), and they weren't offended at all when I misgendered them at first; they corrected me, I apologized, and that was the end of it.
However, if you call the wrong clearly-masculine "alpha male" or clearly-feminine "queen bitch" they/them, you're likely to get a violent reaction.