The main character has a few lines discussing pronouns. And there's the ambiguous gender / cross-gender attractions. Here's a better writeup than I would bother writing :)

https://www.nytimes.com/2023/06/30/movies/nimona-nd-stevenson-profile.html

The graphic novelist ND Stevenson wrote the trans allegory long before he came out. When it was time to adapt it for a new film, he was ready to go further.